Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PBSC collection from G-CSF primed donors.

M Valbonesi1, N Pollicardo, P Carlier

  • 1Immunohematology Service S. Martino Hospital, Genoa, Italy.

Transfusion Science
|November 3, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anaphylaxis Quality of Life Scale for adults: the validation on the Italian allergic patients.

European annals of allergy and clinical immunology·2025
Same author

Direct determination of chronic myeloid leukemia prevalence in Lombardy-Italy: Global implications.

Hematological oncology·2024
Same author

Purtscher-like retinopathy in a paediatric patient with haemolytic uraemic syndrome: A case report and literature review.

Archivos de la Sociedad Espanola de Oftalmologia·2021
Same author

Purtscher-like retinopathy in a paediatric patient with haemolytic uraemic syndrome: A case report and literature review.

Archivos de la Sociedad Espanola de Oftalmologia·2020
Same author

Macular changes in intracranial hypertension.

Archivos de la Sociedad Espanola de Oftalmologia·2020
Same author

The New Challenge of Geriatrics: Saving Frail Older People from the SARS-COV-2 Pandemic Infection.

The journal of nutrition, health & aging·2020

This study compared five cell separators for collecting peripheral blood stem cells (PBSCs). The Excel and MCS 3p machines showed higher efficiency in collecting CD34+ cells for transplantation.

Area of Science:

  • Hematology
  • Transplantation Medicine
  • Biomedical Engineering

Background:

  • Peripheral blood stem cells (PBSCs) are increasingly used in autologous and allogeneic transplantation as an alternative to bone marrow.
  • G-CSF (granulocyte-colony stimulating factor) priming is standard for mobilizing PBSCs from healthy donors.
  • No comparative studies exist on the efficacy of different apheresis machines for PBSC collection.

Purpose of the Study:

  • To prospectively evaluate and compare the efficiency of five different cell separators for PBSC collection.
  • To determine which apheresis machines yield higher quantities and quality of CD34+ cells.

Main Methods:

  • A prospective study randomly assigned 25 donors to different cell separators.
  • Donors received subcutaneous G-CSF for 7 days.

Related Experiment Videos

  • Leukaphereses were performed on days 6 and 7, collecting a total of 50 procedures.
  • Five apheresis machines were used: CS3000 plus, AS 104, Excel, Cobe Spectra, and MCS 3p.

    • Main Results:

    • CD34+ cell yields ranged from 48.44 x 10^6 to 270.37 x 10^6.
    • The Excel and MCS 3p machines demonstrated higher CD34+ cell collection efficiency compared to others.
    • The Excel processed 12 L of blood, while the MCS 3p used an 8 L procedure, potentially influencing observed differences.
    • All machines provided satisfactory yield and quality of harvested PBSCs.

    Conclusions:

    • The Excel and MCS 3p apheresis systems appear more efficient for CD34+ cell collection.
    • Further studies are needed to normalize for processed blood volume when comparing machine efficacy.
    • All evaluated cell separators are capable of collecting adequate PBSCs for transplantation purposes.