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On the use of primary reference grade polydimethylsiloxane
1Department of Bioengineering, Clemson University, South Carolina 29634.
Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials
|April 1, 1993
Summary
Polydimethylsiloxane (PDMS) is increasingly used to study cellular responses to biomaterials. However, this review questions the suitability of in vivo studies using PDMS as a standard reference material due to variations in surface topography.
Area of Science:
- Biomaterials Science
- Biocompatibility Testing
- Cellular Biology
Background:
- Primary reference materials are crucial for standardizing cellular response assessments to biomaterials.
- Polydimethylsiloxane (PDMS), specifically NHLBI-DTB PDMS, is a commonly used biomaterial for blood-contacting studies.
- This material consists of PDMS on a mylar backing.
Purpose of the Study:
- To evaluate the use of NHLBI-DTB PDMS as a primary reference material for biomaterial studies.
- To discuss findings from implantation studies in the context of material variations.
- To question the appropriateness of current in vivo studies employing PDMS.
Main Methods:
- Comparative analysis of two distinct publications on PDMS implantation studies.
- Examination of material composition and surface topography differences.
- Literature review focusing on in vivo cellular response data.
Main Results:
- Significant variations exist in the materials and surface topographies used across different PDMS studies.
- Implantation study results differ based on these material and topographical variations.
- The consistency of PDMS as a standardized reference material is challenged.
Conclusions:
- The current application of PDMS as a universal in vivo reference standard requires critical reassessment.
- Variations in surface topography and material composition impact cellular responses.
- Further standardization or alternative reference materials may be needed for reliable biomaterial research.