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[An enzymic assay for the determination of vitamin B12 (author's transl)]
Hoppe-Seyler'S Zeitschrift Fur Physiologische Chemie
|December 1, 1976
Abstract:
A procedure which extends the very sensitive propanediol-dehydratase reaction for 5'-deoxy-5'-adenosylcobalamine determination to cyanocobalamine and hydroxocobalamine is described. In this procedure, the cobalamines are adenosylated by vitamin B12 coenzyme synthetase and the resulting 5'-deoxy-5'-adenosylcobalamine can be determined by the propanediol-dehydratase reaction. Aldehyde, the product of the latter, can be registered by the alcohol dehydrogenase reaction.