Related Experiment Video
Updated: Aug 14, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Catalytic properties of mitochondrial NADH-ubiquinone reductase (Complex I)
A D Vinogradov1, E V Gavrikova, V G Grivennikova
1Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia. adv@biochem.bio.msu.su
Abstract:
Qualitative and quantitative characteristics of the reactions catalyzed by the most complex and least understood proton translocating unit of the mammalian respiratory chain (NADH-ubiquinone oxidoreductase, Complex I) are described for enzyme preparations differing in degree of resolution--from intact mitochondria to homogeneous small enzyme fragments. Special attention is given to the problems and pitfalls of reliable interpretation of the kinetic analysis of the enzyme activities. Detailed analysis of the problems concerning the slow active/inactive reversible enzyme transition is provided.
Related Concept Videos
Electron Transport Chains
The ETC is comprised of...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
The Supercomplexes in the Crista Membrane
Role of Reduced Coenzymes NADH and FADH₂
Electron Transport Chain: Complex III and IV

