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Updated: Jul 24, 2026

Real-time Cytotoxicity Assays in Human Whole Blood
Published on: November 7, 2014
[Cytotoxic effect of energy supply system inhibitors on cells from Ehrlich ascite carcinoma]
V V Teplova1, P R Makarov, A A Kudriavtsev
1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Abstract:
It was shown that inhibitors of oxidative phosphorylation (cyanide, rotenone, and oligomycin) and very low concentrations of exogenous prooxidants exerted a pronounced cytotoxic effect on Ehrlich ascites carcinoma cells. We propose that cell injury by reactive oxygen forms is the cause of the cytotoxic effect of the studied inhibitors. It was shown via flow cytometry that inhibitors of oxidative phosphorylation and exogenous prooxidants block cell progress in the cell cycle and induce appearance of cells with reduced DNA content.
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