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HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium
R Dei1, E Tortoli, A Bartoloni
1Istituto di Microbiologia, Università di Firenze, Florence, Italy. amcli@unifi.it
Veterinary Microbiology
|April 3, 1999
Summary
High-performance liquid chromatography (HPLC) cannot reliably distinguish Mycobacterium paratuberculosis from Mycobacterium avium. Further research is needed to validate HPLC as a diagnostic tool for mycobacteriology laboratories.
Area of Science:
- Mycobacteriology
- Analytical Chemistry
Background:
- High-performance liquid chromatography (HPLC) is increasingly used in mycobacteriology.
- HPLC has been proposed as a method to differentiate Mycobacterium paratuberculosis from Mycobacterium avium.
- Previous studies indicated no significant difference between M. avium and a M. paratuberculosis reference strain.
Purpose of the Study:
- To investigate the utility of HPLC for distinguishing M. paratuberculosis from M. avium.
- To analyze additional isolates of M. paratuberculosis to confirm earlier findings.
Main Methods:
- Analysis of multiple isolates of M. paratuberculosis and M. avium using HPLC.
- Comparison of chromatographic profiles, specifically focusing on peak clusters.
Main Results:
- No consistent, distinguishing differences were observed in the HPLC profiles between M. avium and M. paratuberculosis isolates.
- The double cluster of peaks generated by HPLC was similar for both species.
Conclusions:
- HPLC, as currently applied, is not a suitable method for reliably distinguishing M. paratuberculosis from M. avium.
- The straightforward use of HPLC for identifying M. paratuberculosis in diagnostic laboratories is questionable.