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Related Experiment Videos

Connexin 43 expression reflects neural crest patterns during cardiovascular development.

K L Waldo1, C W Lo, M L Kirby

  • 1Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, 30912-2640, USA. kwaldo@mail.mcg.edu

Developmental Biology
|April 7, 1999
PubMed
Summary

This study used transgenic mice to map cardiac neural crest cells during cardiovascular development, revealing similarities and differences compared to chick models. The findings suggest this mouse model is valuable for studying mammalian cardiac neural crest functions.

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Area of Science:

  • Developmental Biology
  • Cardiovascular Science
  • Genetics

Background:

  • Cardiac neural crest cells are crucial for cardiovascular development, migrating from pharyngeal arches to the outflow tract.
  • Understanding their precise migration patterns and contributions is essential for identifying causes of congenital heart defects.

Purpose of the Study:

  • To document the pattern of cardiac neural crest cells in the caudal pharyngeal arches and cardiac outflow tract using a transgenic mouse model.
  • To compare cardiac neural crest involvement in outflow septation and aortic arch artery development between mouse and chick embryos.
  • To assess the utility of the transgenic mouse line for studying mammalian cardiac neural crest development.

Main Methods:

  • Utilized transgenic mice with a connexin 43 promoter driving lacZ reporter expression specifically in neural crest cells.

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  • Documented lacZ expression patterns in the cardiac outflow tract and pharyngeal arches.
  • Compared findings with established data from quail-chick chimeras.

    • Main Results:

    • lacZ expression in mice mirrored cardiac neural crest cell patterns observed in chick embryos.
    • Differences were noted in neural crest cell entry pathways into the outflow tract (subendocardial in mouse vs. submyocardial and subendocardial in chick).
    • Differential lacZ staining in the mouse aorta and pulmonary trunk suggests potential species-specific differences in neural crest contribution or distribution.

    Conclusions:

    • The transgenic mouse model accurately represents cardiac neural crest cell locations.
    • Similarities suggest conserved functions of cardiac neural crest cells in mammals and birds.
    • Observed differences highlight potential species-specific variations in cardiovascular and neural crest development, warranting further investigation with independent markers.