Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B cell maturation in relation to multiple myeloma.

F K Stevenson1, S S Sahota

  • 1Molecular Immunology Group, Tenovus Laboratory, Southampton University Hospitals, UK.

Pathologie-Biologie
|April 8, 1999
PubMed
Summary

Immunoglobulin variable (V) gene sequencing reveals B-cell tumor clonal history and differentiation. This analysis aids in diagnosing and understanding B-cell malignancies, potentially guiding new therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early stages in the ontogeny of small B-cell lymphomas: genetics and microenvironment.

Journal of internal medicine·2017
Same author

IL-10 production by CLL cells is enhanced in the anergic IGHV mutated subset and associates with reduced DNA methylation of the IL10 locus.

Leukemia·2016
Same author

Phenotypic heterogeneity in IGHV-mutated CLL patients has prognostic impact and identifies a subset with increased sensitivity to BTK and PI3Kδ inhibition.

Leukemia·2014
Same author

Idiotypes and diseases.

Immunology today·2014
Same author

Chronic lymphocytic leukaemia and normal B lymphopoiesis.

Immunology today·2014
Same author

Exome sequencing in tracking clonal evolution in multiple myeloma following therapy.

Leukemia·2012

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • B-cell maturation involves immunoglobulin variable (V) gene rearrangement and somatic mutation.
  • Neoplastic transformation of B cells retains a genetic imprint of their clonal history.

Purpose of the Study:

  • To analyze V-gene sequences in B-cell tumors to understand their origin and evolution.
  • To investigate the role of antigen selection and somatic hypermutation in B-cell malignancies.

Main Methods:

  • Sequence analysis of immunoglobulin variable (V) region genes.
  • Comparison of tumor V-gene sequences with the normal B-cell repertoire.

Main Results:

  • V-gene analysis can identify antigen selection and ongoing somatic mutation in B-cell tumors.
  • Myeloma cells likely originate from antigen-selected plasma cells, potentially with coexisting less mature B cells.
  • Benign plasma cell tumors may contain B cells actively undergoing somatic mutation.

Conclusions:

  • V-gene analysis provides insights into the cellular origin and differentiation pathways of B-cell tumors.
  • This approach aids in diagnosing and monitoring B-cell malignancies.
  • Understanding V-gene dynamics may lead to targeted therapeutic strategies for B-cell cancers.

Related Experiment Videos