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A sensitive and specific ELISA for plasma pentosidine.

Y Izuhara1, T Miyata, Y Ueda

  • 1Molecular and Cellular Nephrology, Institute of Medical Sciences and Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|April 8, 1999
PubMed
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A new enzyme-linked immunosorbent assay (ELISA) accurately measures plasma pentosidine, a marker linked to dialysis complications. This method is faster and more convenient than current assays for assessing carbonyl stress in kidney failure patients.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Nephrology

Background:

  • Advanced glycation end products (AGEs) arise from non-enzymatic glycation and oxidation.
  • Pentosidine, a specific AGE, is implicated in complications of chronic kidney disease (CKD) and dialysis, including amyloidosis and atherosclerosis.

Purpose of the Study:

  • To develop and validate a sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) for quantifying plasma pentosidine.
  • To compare the performance of the developed ELISA with the established high-performance liquid chromatography (HPLC) method.

Main Methods:

  • A competitive ELISA was established for plasma pentosidine detection.
  • The ELISA was applied to plasma samples from hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients.

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  • Results from ELISA were compared with those obtained from HPLC analysis.

    • Main Results:

    • Plasma pentosidine levels measured by ELISA strongly correlated with HPLC assay results in both diabetic and non-diabetic dialysis patients.
    • Both methods showed significantly elevated plasma pentosidine levels (over 8-fold) in HD and CAPD patients compared to healthy individuals and non-uraemic diabetic patients.
    • The presence or absence of diabetes did not significantly alter the elevated pentosidine levels in dialysis patients.

    Conclusions:

    • The developed competitive ELISA offers a rapid, convenient, and reliable method for determining plasma pentosidine.
    • This assay is valuable for assessing carbonyl stress in uraemic patients.
    • The findings highlight elevated pentosidine levels as a common feature in patients undergoing long-term dialysis.