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Functional and structural properties of urinary tissue factor.

B A Lwaleed¹, P S Bass, J L Francis

¹University Department of Haematology, Southampton University Hospitals, UK.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association

|April 8, 1999

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Urinary tissue factor (uTF) is found on subcellular particles originating from renal tubular cells, providing lipid for its function. This study clarifies the structure and origin of uTF in urine.

Area of Science:

Nephrology
Hematology
Cell Biology

Background:

Previous studies highlighted the clinical significance of urinary tissue factor (uTF) in glomerulonephritis and malignancy.
The functional and structural properties, along with the cell of origin for uTF, remain poorly understood.

Purpose of the Study:

To investigate the functional and structural properties of urinary tissue factor (uTF).
To determine the putative cell of origin for uTF found in urine.

Main Methods:

Utilized kinetic chromogenic assay, ELISA, and transmission electron microscopy (TEM) on urine from 69 healthy controls.
Employed immunoperoxidase staining for tissue factor (TF) antigen and Annexin V on kidney sections.

Main Results:

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TEM revealed uTF on subcellular particles containing anionic phospholipid, indicated by Annexin V binding.
TF antigen and Annexin V were detected in the cytoplasm of renal proximal and distal tubules, but not glomerular cells.

Conclusions:

Urinary tissue factor (uTF) is associated with subcellular particles that supply lipids essential for its activity.
Both uTF and its associated vesicles originate from renal tubular cells.