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Related Concept Videos

Generation of Action Potential in Skeletal Muscles01:24

Generation of Action Potential in Skeletal Muscles

Every cell in the body maintains a membrane potential due to an uneven distribution of positive and negative charges across its plasma membrane. The membrane potential is measured in millivolts and quantifies the difference in charge across the membrane.
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the cell's...

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Electroporation of Plasmid DNA into Mouse Skeletal Muscle
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Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cells.

M Bier1, S M Hammer, D J Canaday

  • 1Department of Organismal Biology and Anatomy, Pritzker School of Medicine, University of Chicago, Illinois, USA. mbier@surgery.bsd.uchicago.edu

Bioelectromagnetics
|April 9, 1999
PubMed
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Electroporation creates temporary or stable plasma membrane pores. Researchers found transient electroporation pore sealing takes about 9 minutes in skeletal muscle cells, regardless of electrical potential.

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Area of Science:

  • Cellular biology
  • Biophysics
  • Electrophysiology

Background:

  • Electroporation involves plasma membrane permeabilization via electrical forces.
  • Pore formation is thought to initiate in the lipid bilayer.
  • Understanding pore sealing kinetics is crucial for electroporation applications.

Purpose of the Study:

  • To determine the time constants for spontaneous transient pore sealing after electroporation.
  • To identify the transmembrane potential thresholds for transient and stable electroporation in skeletal muscle cells.

Main Methods:

  • Isolated rat flexor digitorum brevis skeletal muscle cells were used.
  • A two-compartment diffusion model was employed to analyze pore sealing.
  • Transmembrane potential thresholds were systematically varied.

Main Results:

  • Transient electroporation pore sealing time (τp) was approximately 9 minutes.
  • Pore sealing time was independent of the applied transmembrane potential.
  • Transient pores formed at 340-480 mV, while stable pores formed at ≥540 mV, indicated by magnesium influx and equilibration.

Conclusions:

  • Spontaneously sealing pores in skeletal muscle cells have a characteristic sealing time of ~9 minutes.
  • Higher electrical potentials are required to induce stable, long-lasting membrane permeabilization.
  • Findings aid understanding of electrical injury and optimizing electroporation for gene transfection and cell transformation.