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Short communication: lack of stage-specific embryonic antigen-1 expression by bovine embryos and primordial germ
S J Choi1, H Shim, G B Anderson
1Department of Animal Science, University of California, Davis 95616, USA.
Abstract:
The objective of this study was to determine whether stage-specific embryonic antigen-1, a cellular marker commonly used to identify murine undifferentiated embryonic cells, is also a useful marker for bovine pluripotent cells. Expression of stage-specific embryonic antigen-1 was examined by indirect immunohistochemistry on bovine preimplantation embryos and on primordial germ cells contained in the genital ridge. Expression of stage-specific embryonic antigen-1 was not observed in any of the cleavage-stage bovine embryos examined, including one-cell, two-cell, four-cell, eight-cell, morula, and blastocyst stages, nor in tissue sections of bovine genital ridges collected from embryos on d 34, 37, and 40 of gestation. As expected, expression of stage-specific embryonic antigen-1 was detected on murine preimplantation embryos and on murine teratocarcinoma cells. Results of this study indicate that, unlike in the mouse, stage-specific embryonic antigen-1 is not a useful cellular marker for pluripotent bovine embryonic cells or bovine primordial germ cells.