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Related Experiment Videos

Lymphoblastoid cell lines with integrated human herpesvirus type 6.

M Daibata1, T Taguchi, I Kubonishi

  • 1Department of Medicine, Kochi Medical School, Japan. daibatam@kochi-ms.ac.jp

Journal of Human Virology
|April 9, 1999
PubMed
Summary

Stable cell lines latently infected with human herpesvirus type 6 (HHV-6) were established. These HHV-6-positive lymphoblastoid cell lines are valuable for studying viral integration mechanisms.

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Area of Science:

  • Virology
  • Cell Biology
  • Genetics

Background:

  • Human herpesvirus type 6 (HHV-6) is a common human virus.
  • Establishing in vitro models for latent HHV-6 infection is crucial for understanding its pathogenesis.
  • Previous studies focused on HHV-6 in specific patient populations.

Purpose of the Study:

  • To establish stable in vitro cell lines latently infected with HHV-6.
  • To investigate the integration site and latency of HHV-6 in immortalized cells.
  • To create a resource for studying HHV-6 integration mechanisms.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) from an HHV-6 infected patient were immortalized using Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS).
  • Polymerase chain reaction (PCR) and Southern blot hybridization confirmed HHV-6 DNA presence.

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  • Fluorescence in situ hybridization (FISH) identified the HHV-6 integration site on chromosome 1 (1q44).

    • Main Results:

    • B- and T-lymphoblastoid cell lines latently infected with HHV-6 were successfully established.
    • HHV-6 DNA was detected in both cell lines, with integration confirmed at chromosome 1q44.
    • Absence of HHV-6 immediate-early gene transcripts and virion-associated protein indicated a latent infection state.

    Conclusions:

    • The developed HHV-6-positive lymphoblastoid cell lines provide a stable in vitro model for latent HHV-6 infection.
    • These cell lines are instrumental for elucidating the molecular mechanisms of HHV-6 DNA integration.
    • The findings facilitate further research into HHV-6 persistence and associated diseases.