Related Experiment Video

Updated: Aug 4, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Cellular response to DNA damage. Link between p53 and DNA-PK

I Salles-Passador1, A Fotedar, R Fotedar

  • 1Institut de biologie structurale J.-P.-Ebel, Grenoble, France.

Comptes Rendus De L'Academie Des Sciences. Serie III, Sciences De La Vie
|April 10, 1999
PubMed

Insights

Cells lacking DNA-activated protein kinase (DNA-PK) cannot repair DNA double-strand breaks and are sensitive to radiation. DNA-PK may interact with p53, influencing cellular growth and radiation response.

Area of Science:

  • Cellular biology
  • Molecular genetics
  • Radiation oncology

Background:

  • DNA-activated protein kinase (DNA-PK) is crucial for DNA double-strand break repair.
  • Cells deficient in DNA-PK exhibit extreme sensitivity to ionizing radiation.
  • DNA-PK belongs to a kinase family including ATR and ATM, sharing homology with PL-3 kinase.

Purpose of the Study:

  • To investigate the role of DNA-PK in cellular response to DNA damage.
  • To explore the potential interaction between DNA-PK and p53 in radiation response and cell growth control.

Main Methods:

  • Comparative analysis of DNA repair capabilities in cells with and without DNA-PK.
  • Examination of protein kinase family homology (DNA-PK, ATR, ATM, PL-3 kinase).
  • Hypothesizing the involvement of DNA-PK in p53 signaling pathways.

Main Results:

  • Cells lacking DNA-PK are highly susceptible to ionizing radiation.
  • These cells demonstrate an impaired ability to repair DNA double-strand breaks.
  • ATM, a related kinase, is known to function upstream of p53 in radiation response.

Conclusions:

  • DNA-PK plays a critical role in repairing DNA double-strand breaks and mitigating radiation sensitivity.
  • DNA-PK's potential interaction with p53 suggests a role in regulating cell growth and mediating cellular responses to ionizing radiation.

More Related Videos

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...