Related Experiment Video
Updated: Sep 10, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
Published on: July 17, 2020
SHIP is a negative regulator of growth factor receptor-mediated PKB/Akt activation and myeloid cell survival
Q Liu1, T Sasaki, I Kozieradzki
1Amgen Institute, Toronto, Ontario, Canada M5G 2C1.
Abstract:
SHIP is an inositol 5' phosphatase that hydrolyzes the PI3'K product PI(3,4,5)P3. We show that SHIP-deficient mice exhibit dramatic chronic hyperplasia of myeloid cells resulting in splenomegaly, lymphadenopathy, and myeloid infiltration of vital organs. Neutrophils and bone marrow-derived mast cells from SHIP-/- mice are less susceptible to programmed cell death induced by various apoptotic stimuli or by growth factor withdrawal. Engagement of IL3-R and GM-CSF-R in these cells leads to increased and prolonged PI3'K-dependent PI(3,4,5)P3 accumulation and PKB activation. These data indicate that SHIP is a negative regulator of growth factor-mediated PKB activation and myeloid cell survival.
Related Concept Videos
Negative Regulator Molecules
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Regulation of Angiogenesis and Blood Supply
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway

