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Related Experiment Videos

Cyclic AMP can decrease expression of genes subject to catabolite repression in Saccharomyces cerevisiae.

O Zaragoza1, C Lindley, J M Gancedo

  • 1Instituto de Investigaciones Biomédicas "Alberto Sols," CSIC-UAM, 28029 Madrid, Spain.

Journal of Bacteriology
|April 10, 1999
PubMed
Summary
This summary is machine-generated.

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External cyclic AMP (cAMP) inhibits gluconeogenic enzyme gene expression in yeast by reducing transcription. This regulation of fructose-1,6-bisphosphatase (FBP1) gene transcription by cAMP is redundant with other control mechanisms.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Gene Regulation

Background:

  • Cyclic AMP (cAMP) is a crucial second messenger regulating various cellular processes.
  • Understanding cAMP's role in gene expression is vital for deciphering metabolic control in yeast.
  • The FBP1 gene encodes fructose-1,6-bisphosphatase, a key gluconeogenic enzyme.

Purpose of the Study:

  • To investigate the effect of external cAMP on the derepression of gluconeogenic enzymes in Saccharomyces cerevisiae.
  • To elucidate the regulatory mechanisms by which cAMP controls FBP1 gene transcription.
  • To determine if cAMP-mediated regulation involves the Mig1 repressor protein.

Main Methods:

  • Yeast genetics (pde2 mutant strain)
  • Transcriptional analysis using reporter constructs (UAS1FBP1 and UAS2FBP1)

Related Experiment Videos

  • Electrophoretic mobility shift assays (EMSA) to study protein-DNA interactions

    • Main Results:

    • External cAMP significantly hindered gluconeogenic enzyme derepression in a pde2 mutant.
    • cAMP reduced FBP1 transcription by approximately 20-fold (UAS1FBP1) and 2-fold (UAS2FBP1).
    • Nuclear extracts showed impaired formation of UASFBP1-protein complexes in the presence of cAMP, suggesting interference with transcription factor binding.

    Conclusions:

    • External cAMP negatively regulates FBP1 gene transcription in yeast.
    • The mechanism involves impaired binding of transcription factors to the FBP1 upstream activation sequences.
    • cAMP-mediated control of FBP1 transcription is redundant with other regulatory pathways and does not appear to involve the Mig1 repressor.