Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oxygen requirement for prostaglandin biosynthesis.

W E Lands, J Sauter, G W Stone

    Prostaglandins and Medicine
    |August 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The cool brown dwarf Gliese 229 B is a close binary.

    Nature·2024
    Same author

    Eleventh Annual Meeting of the Michigan Dental Association.

    The Dental register·2021
    Same author

    Annual Meeting of the Michigan Dental Association.

    The Dental register·2021
    Same author

    Address before the Michigan Dental Association.

    The Dental register·2021
    Same author

    Blood group A epitopes do not facilitate entry of SARS-CoV-2.

    Journal of internal medicine·2021
    Same author

    HLA-E typing of more than 2.5 million potential hematopoietic stem cell donors: Methods and population-specific allele frequencies.

    Human immunology·2021

    Prostaglandin production by cyclooxygenase is sensitive to oxygen levels. Most tissues have enough oxygen, but some may experience low oxygen, limiting prostaglandin synthesis.

    Area of Science:

    • Biochemistry
    • Physiology

    Background:

    • Prostaglandins are crucial lipid compounds involved in various physiological processes.
    • Cyclooxygenase (COX) enzymes are key in prostaglandin biosynthesis.
    • Oxygen (O2) availability is a potential regulatory factor in prostaglandin formation.

    Purpose of the Study:

    • To investigate the effect of oxygen concentrations on prostaglandin formation by vesicular gland cyclooxygenase.
    • To determine the apparent Michaelis constant (Km) for oxygen in this process.

    Main Methods:

    • Enzymatic assays measuring prostaglandin synthesis.
    • Vesicular gland cyclooxygenase was used as the enzyme source.
    • Varying oxygen concentrations were applied to the reaction.

    Related Experiment Videos

    Main Results:

    • Prostaglandin formation was regulated by O2 concentrations below 30 micron (20 mm Hg).
    • The apparent Km for O2 was determined to be approximately 5 micron.
    • This indicates a significant sensitivity of the enzyme to oxygen levels.

    Conclusions:

    • Most mammalian tissues likely maintain sufficient O2 levels for substantial prostaglandin biosynthesis.
    • Under certain physiological conditions, oxygen availability may become a limiting factor for prostaglandin synthesis in some tissues.
    • The findings highlight the importance of local oxygen tension in regulating inflammatory and physiological responses mediated by prostaglandins.