Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analyte comparisons between 2 clinical chemistry analyzers.

A Sutton1, H Dawson, B Hoff

  • 1Animal Health Laboratory, University of Guelph, Ontario.

The Canadian Veterinary Journal = La Revue Veterinaire Canadienne
|April 14, 1999
PubMed
Summary

This study compared wet and dry reagent analyzers for veterinary serum analytes. Dry analyzers showed excellent agreement for most tests, but reliability varied for albumin, potassium, and calcium.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision Measurement of Neutrino Oscillation Parameters with 10 Years of Data from the NOvA Experiment.

Physical review letters·2026
Same author

Mortality and Major Amputation in Patients With Diabetes-Related Foot Ulcers and Chronic Renal Disease.

ANZ journal of surgery·2025
Same author

Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

Physical review letters·2025
Same author

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

Physical review letters·2024
Same author

Measurement of the ν_{e}-Nucleus Charged-Current Double-Differential Cross Section at ⟨E_{ν}⟩=2.4  GeV Using NOvA.

Physical review letters·2023
Same author

Search for Active-Sterile Antineutrino Mixing Using Neutral-Current Interactions with the NOvA Experiment.

Physical review letters·2021

Area of Science:

  • Veterinary Clinical Pathology
  • Analytical Chemistry

Background:

  • Accurate measurement of serum analytes is crucial for veterinary diagnostics.
  • Wet reagent analyzers are common, but dry reagent systems offer potential advantages in convenience and speed.

Purpose of the Study:

  • To evaluate the agreement between a wet reagent analyzer and a dry reagent analyzer for thirteen common veterinary serum analytes in canine and feline samples.
  • To identify specific analytes and species where the dry reagent analyzer demonstrates reliable performance compared to the established wet reagent method.

Main Methods:

  • Comparison of thirteen serum analytes (albumin, globulin, alkaline phosphatase, alanine aminotransferase, amylase, urea nitrogen, calcium, cholesterol, creatinine, glucose, potassium, total bilirubin, and total protein) using both wet and dry reagent analyzers.

Related Experiment Videos

  • Data analysis employed concordance correlation coefficients, linear regression, and Bland-Altman plots to assess agreement and identify biases.

    • Main Results:

    • Excellent agreement (concordance correlation coefficient r > or = 0.90) was observed for 8 out of 13 analytes.
    • Dry reagent analyzer showed excellent agreement for alkaline phosphatase, alanine aminotransferase, amylase (feline), urea nitrogen, cholesterol, creatinine, glucose, total bilirubin (canine), and total protein.
    • Clinically unreliable agreement was noted for albumin, potassium, and calcium, with slight to substantial agreement for amylase (canine) and total bilirubin (feline).
    • Linear regression indicated methodological differences, with several analytes showing slopes significantly deviating from unity.

    Conclusions:

    • Dry reagent analyzers provide excellent agreement for a majority of tested veterinary serum analytes, particularly in canine samples.
    • Specific analytes like albumin, potassium, and calcium require cautious interpretation when using the dry reagent analyzer due to unreliable agreement.
    • Further investigation into methodological differences may optimize the performance of dry reagent analyzers in veterinary diagnostics.