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Related Experiment Videos

CREB-mediated transcriptional control.

O M Andrisani1

  • 1Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907-1246, USA.

Critical Reviews in Eukaryotic Gene Expression
|April 14, 1999
PubMed
Summary

The cAMP-response-element-binding protein (CREB) regulates gene transcription through phosphorylation and interaction with co-activators like CBP. This transcription factor plays a key role in cellular growth and development.

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cellular Signaling

Background:

  • cAMP-response-element-binding protein (CREB) is a transcription factor crucial for regulating cAMP-inducible genes.
  • CREB functions as a homodimer, binding to cAMP-response elements (CREs) via its leucine zipper motif.
  • Transcriptional activity is modulated by phosphorylation at Ser133 and interaction with CREB-binding protein (CBP).

Purpose of the Study:

  • To review key experiments defining CREB activation mechanisms.
  • To highlight the role of CREB in cellular growth and development.
  • To discuss alternative CREB regulation by viral oncoproteins.

Main Methods:

  • Literature review of experimental studies on CREB.
  • Analysis of CREB structure, function, and transcriptional regulation.
  • Examination of signaling pathways affecting CREB activity.

Main Results:

  • CREB activation involves phosphorylation at Ser133, leading to CBP recruitment and gene transcription.
  • Diverse signals, including cAMP, Ca+2, growth factors, and stress, modulate CREB activity.
  • Viral oncoproteins (HBV pX, HTLV-I Tax) enhance CREB DNA-binding affinity for viral and cellular CRE sites.

Conclusions:

  • CREB-mediated transcription regulates critical cellular processes like metabolism, neuronal signaling, proliferation, and apoptosis.
  • Phosphorylation-dependent and independent mechanisms regulate CREB's transcriptional role.
  • Emerging evidence strongly links CREB to cellular growth and developmental processes.

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