Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-dependent mechanics of single DNA molecules.

M Rief1, H Clausen-Schaumann, H E Gaub

  • 1Lehrstuhl für Angewandte Physik, München, Germany.

Nature Structural Biology
|April 14, 1999
PubMed
Summary

Atomic force microscopy reveals DNA

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aortic aneurysm and aortic graft infection related to Mycobacterium bovis after intravesical Bacille Calmette-Guérin therapy-a case series.

BMC surgery·2021
Same author

The Matrilin-3 T298M mutation predisposes for post-traumatic osteoarthritis in a knock-in mouse model.

Osteoarthritis and cartilage·2020
Same author

[System and skill utilization in an Austrian emergency physician system: retrospective study].

Der Anaesthesist·2020
Same author

Temporal averaging for analysis of four-dimensional whole-heart computed tomography perfusion of the myocardium: proof-of-concept study.

The international journal of cardiovascular imaging·2016
Same author

[Erratum to: Teleradiological report turnaround times. An internal efficiency and quality control analysis].

Der Radiologe·2015
Same author

[Teleradiological report turnaround times: An internal efficiency and quality control analysis].

Der Radiologe·2015

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • DNA's mechanical properties are crucial for biological functions.
  • Understanding sequence-dependent mechanics informs DNA-protein interactions and drug design.

Purpose of the Study:

  • To investigate sequence-dependent mechanical properties of DNA using single-molecule force spectroscopy.
  • To characterize the B-S transition and a novel melting transition in DNA.
  • To determine base pair unbinding forces for G-C and A-T pairs.

Main Methods:

  • Atomic force microscope (AFM)-based single-molecule force spectroscopy.
  • Stretching individual DNA double strands.
  • Comparative analysis of lambda-phage DNA, poly(dG-dC), and poly(dA-dT).

Main Results:

  • Identified a B-S transition at 65 pN and a nonequilibrium melting transition at 150 pN in lambda-phage DNA.
  • Melting transition involves DNA splitting into single strands and subsequent recombination.
  • Poly(dA-dT) exhibited lower transition forces compared to poly(dG-dC).
  • Determined G-C base pair unbinding force as 20 ± 3 pN and A-T as 9 ± 3 pN.

Conclusions:

  • DNA exhibits distinct mechanical transitions, including a novel melting transition.
  • Sequence significantly influences DNA's mechanical behavior.
  • AFM-based force spectroscopy provides precise measurements of base pair stability.

Related Experiment Videos