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Disk diffusion method for determining susceptibilities of Candida spp. to MK-0991
M Lozano-Chiu1, P W Nelson, V L Paetznick
1Division of Infectious Diseases, Department of Internal Medicine, Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030, USA. mlc34906@Glaxo.Wellcome.co.uk
Journal of Clinical Microbiology
|April 16, 1999
Summary
A new agar-based method effectively tests Candida susceptibility to antifungal MK-0991, a glucan synthase inhibitor. Results align with standard broth methods, though some variations require further in vivo investigation.
Area of Science:
- Mycology
- Antimicrobial Susceptibility Testing
- Pharmacology
Background:
- Candida species pose significant threats, necessitating effective antifungal treatments.
- Glucan synthase inhibitors represent a novel class of antifungal agents.
- Standardized methods for assessing antifungal susceptibility are crucial for clinical decision-making.
Purpose of the Study:
- To develop and validate an agar-based methodology for evaluating Candida susceptibility to MK-0991.
- To compare the performance of the novel agar-based method with a standard broth microdilution reference method.
Main Methods:
- Development of an agar-based susceptibility testing protocol for Candida isolates.
- Testing of Candida spp. against the antifungal agent MK-0991 using the developed agar method.
- Comparison of results with the National Committee for Clinical Laboratory Standards M27-A broth microdilution reference method.
Main Results:
- The agar-based methodology demonstrated good correlation with the M27-A broth microdilution reference method.
- Some Candida isolates exhibited inhibition zones on agar that were inconsistent with their determined Minimum Inhibitory Concentrations (MICs).
Conclusions:
- The developed agar-based method shows promise for assessing Candida susceptibility to MK-0991.
- Discrepancies between agar inhibition zones and broth MICs highlight the need for further in vivo studies to fully understand the clinical implications.