Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polarization sensing with visual detection.

I Gryczynski1, Z Gryczynski, J R Lakowicz

  • 1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.

Analytical Chemistry
|April 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoselection of Luminescent Molecules in Anisotropic Media in the Case of Two-Photon Excitation. Part II. Experimental Studies of Hoechst 33342 in Stretched Poly(vinyl alcohol) Films.

Zeitschrift fur Naturforschung. A, Journal of physical sciences·2021
Same author

Site-to-site distance distribution in flexible molecules: theoretical evaluation of the donor and/or acceptor fluorescence decay function.

Journal of luminescence·2021
Same author

Correction to: Transamidase site-targeted agents alter the conformation of the transglutaminase cancer stem cell survival protein to reduce GTP binding activity and cancer stem cell survival.

Oncogene·2021
Same author

Time-Resolved Fluorescence Intensity and Anisotropy Decays of 2,5-Diphenyloxazole by Two-Photon Excitation and Frequency-Domain Fluorometry.

The Journal of physical chemistry·2019
Same author

Photophysical properties of 2-Phenylindole in poly (vinyl alcohol) film at room temperature. Enhanced phosphorescence anisotropy with direct triplet state excitation.

Methods and applications in fluorescence·2019
Same author

ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.

Acta physica Polonica: A·2019
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

This study introduces a novel visual fluorescence sensing method using polarization changes. This simple, electronic-free device allows for accurate analyte detection, including pH and concentration measurements.

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Materials Science

Background:

  • Fluorescence sensing is crucial for detecting analytes.
  • Existing methods often require complex instrumentation.
  • Visual detection methods offer simplicity and accessibility.

Purpose of the Study:

  • To develop a new, visually-based fluorescence sensing approach.
  • To demonstrate the versatility of this method for different analytes.
  • To create a simple, low-cost sensing device.

Main Methods:

  • Utilizing a fluorescent probe whose intensity changes with analyte concentration.
  • Employing an oriented fluorescent film as a reference.
  • Measuring changes in the polarization of combined emissions visually.

Related Experiment Videos

  • Using a dual polarizer and an analyzing polarizer for detection.

    • Main Results:

    • Successfully measured Rhodamine B (RhB) concentration in intralipid.
    • Accurately determined pH using 6-carboxyfluorescein, with +/- 0.1 pH unit accuracy.
    • Developed a film-free method using the same fluorophore for sample and reference.

    Conclusions:

    • The described visual polarization sensing is a generic and robust technique.
    • The method is simple, requires minimal electronic components, and is cost-effective.
    • This approach has broad applicability for various analytes with available fluorescent probes.