The escape of bile from the intrhepatic biliary tree in acute bile stasis

The escape of fluorescent dyes from the bile passages during bile stasis and after a retrograde infusion into the common bile duct was examined in rats and mice. After an intravenous dye injection in bile stasis a strong fluorescence is seen in the periportal spaces. During retrograde dye infusion great amounts of dye accumulate in the periportal spaces but, in consequence of focal disruption of liver cell membranes, escapes also into the spaces of Disse, to be consequently transported by the sinusoidal blood.