[External quality control of the laboratory determination of glucose in the urine and material for its realization]

Urine of diabetics without concomitant diseases with a certain concentration of glucose was collected for preparing reference material. Water saturated with phenol (872 mmole/liter) was added to urine pool during intensive stirring on a magnetic stirrer at 20 degrees C, so that the final concentration of phenol were 50 mmole/liter. Glucose content in the resultant reference material remained stable for 130 days at 18-20 degrees C. For glucose measurements, 2 ml reference material and 0.5 ml 2.5 M sodium oxide hydrate are put in centrifuge tubes, stirred, put on boiling water bath for 2 min, cooled, and colorimetried in cuvettes with 1-mm optic route. The content of monosaccharide is calculated using the proportion rule in comparison with the values for 139 mM reference glucose solution in control urine.