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High level expression of soluble angiogenin in Escherichia coli
1Department of Microbial Chemistry, College of Pharmacy, Seoul National University, Kwanak-gu.
Biochemistry and Molecular Biology International
|April 17, 1999
Abstract:
Human angiogenin was genetically engineered and contained the E. coli Omp A signal sequence for secreting soluble angiogenin to the periplasm under tac promoter control. The angiogenin sequence was encoded in a single gene and expressed as a 14.4 kilodalton soluble protein in E. coli. It was purified by CM-Sepharose ion-exchange chromatography and by a heparin-Sepharose affinity chromatography procedure. The biological activity of angiogenin was established by its ability to inhibit mRNA-dependent rabbit reticulocyte cell-free translation.