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Antifungal activity of chlorhexidine containing mouthrinses. An in vitro study
1Cattedra di Parodontologia, Università degli Studi, Palermo.
Background:
Oral candidosis has emerged as an increasing problem in immunocompromised patients because oral cavity represents an important port of entry for systemic fungal infections.
Methods:
The aim of the present study was to investigate the in vitro antifungal activity of 5 commercial mouthrinses containing chlorhexidine (CHX). The Minimum Fungicidal Concentration (MFC) against six species of yeasts was determined by a broth macrodilution method. The kill-time of mouthrinses at half the concentration of the commercial formulations was also determined.
Results:
MFCs were achieved with each mouthrinses against all the organisms under test. However, significant differences in MFC values were found for Ebur Os in comparison with Dentosan, Corsodyl and Plak out (p < 0.001). Kill-times of Corsodyl and Dentosan were less than or equal to 120 sec with all the species of yeasts, except Torulopsis glabrata. Significant differences were found in kill-time values between Dentosan and Broxo Din only (p < 0.001).
Conclusions:
These results suggest that CHX-containing mouthrinses may represent an appropriate alternative to conventional antifungal drugs in the management of oral candidosis. Effectiveness in preventing systemic fungal infections in immunocompromised patients requires further in vivo studies.
Insights
Five commercial mouthrinses containing chlorhexidine (CHX) showed antifungal activity against oral yeasts. Some CHX mouthrinses demonstrated faster kill-times, suggesting potential for managing oral candidosis.
Area of Science:
- Oral microbiology
- Mycology
- Pharmacology
Background:
- Oral candidosis is a growing concern in immunocompromised individuals.
- The oral cavity serves as a potential entry point for systemic fungal infections.
Purpose of the Study:
- To evaluate the in vitro antifungal efficacy of five commercial mouthrinses containing chlorhexidine (CHX).
- To determine the Minimum Fungicidal Concentration (MFC) and kill-time of CHX mouthrinses against various yeast species.
Main Methods:
- In vitro antifungal activity testing of five commercial mouthrinses containing chlorhexidine (CHX).
- Broth macrodilution method used to determine Minimum Fungicidal Concentration (MFC) against six yeast species.
- Kill-time assays performed at half the commercial concentration of the mouthrinses.
Main Results:
- All tested CHX mouthrinses exhibited fungicidal activity against the tested yeast species.
- Significant differences in MFC values were observed between certain mouthrinse brands.
- Corsodyl and Dentosan showed kill-times under 120 seconds for most yeasts, with significant differences noted between Dentosan and Broxo Din.
Conclusions:
- Chlorhexidine (CHX)-containing mouthrinses show promise as an alternative treatment for oral candidosis.
- Further in vivo research is necessary to confirm their effectiveness in preventing systemic fungal infections in immunocompromised patients.