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Updated: Jul 24, 2026

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
Published on: December 19, 2010
Acid and base regulation in the proteome of Escherichia coli
J L Slonczewski1, D Blankenhorn
1Department of Biology, Kenyon College, Gambier, OH 43022, USA.
Abstract:
Acid and base conditions have many significant effects on the growth of Escherichia coli. External and internal pH perturbations induce different classes of genes. pH-dependent regulation of genes intersects with other regulatory responses, e.g. oxygen level or osmolarity. 2D electrophoretic gels were used to compare global patterns of protein induction in Escherichia coli grown in complex media buffered at the acid or alkaline ends of the pH range for growth (pH 4.4 vs. pH 9.1). Preliminary results indicate new classes of acid- and base-dependent regulation, in some cases highly dependent on oxygen level. Other proteins are induced strongly at both extremes of pH, compared to pH 7. Current work continues to dissect the relationship between effects of pH, oxygen level and osmolarity.
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