Cation movements at alkaline pH in bacteria growing without respiration

The respiratory chain has a central role in energy metabolism as a generator of a proton motive force in aerobic bacteria. In contrast, Enterococcus hirae (formerly Streptococcus faecalis), which lacks the respiratory chain, generates this proton gradient via a F-type H+ ATPase, but it works only at a pH below 8; no significant proton motive force is generated at a pH above 8. An Escherichia coli mutant deficient in both the respiratory chain and the H+ ATPase grew with a negligible proton motive force within the wide range of medium pH. It has been suggested that both E. hirae and E. coli are able to grow even when the cytoplasm is alkalinized beyond pH 8. These observations lead to the conclusion that bacteria can survive without operating cation transport systems driven by a proton motive force at alkaline pH. The activity of any transport system with optimum pH around neutrality should decline when both the outside and inside of cells are alkalinized. Thus, changes in transport systems as well as cellular metabolism may be essential for bacterial adaptation to changes in environmental pH.