Identification of a novel gene product preferentially expressed in rat lens epithelial cells

¹Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, NJ 07103, USA. hui_cai@bausch.com

|April 21, 1999

Abstract

Purpose:

We searched for an mRNA that is differentially expressed in rat lens epithelial and fiber cells to use as a marker for differentiation.

Methods:

An mRNA differential display method was used to identify genes with altered expression during differentiation of lens epithelial cells.

Results:

One gene (partial sequence) was identified and confirmed to be expressed preferentially in undifferentiated rat lens epithelial cells. Among non-lens tissues, a trace amount was detected in cornea, but not in retina or eleven non-ocular tissues tested. This cloned partial gene sequence (322 base pairs) is at least 93% identical to overlapping rat and mouse EST sequences, and extends the sequence of both ESTs at the 5' end.

Conclusions:

A clone has been isolated that is preferentially expressed in lens epithelial cells. Expression of this gene was down-regulated when rat pup lens epithelial cell explants were induced to differentiate. The unusual pattern of expression suggests a novel function for this gene.

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Identification of a novel gene product preferentially expressed in rat lens epithelial cells

Purpose:

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Conclusions:

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