Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Infrequent bax gene mutations in B-cell lymphomas.

H Peng1, A Aiello, G Packham

  • 1Department of Histopathology, UCL Medical School, London, U.K.

The Journal of Pathology
|April 21, 1999
PubMed
Summary

Bax gene mutations are uncommon in B-cell lymphomas. This study investigated bax gene alterations in 112 B-cell lymphoma cases, finding few tumor-specific mutations, suggesting apoptosis inhibition via bax is not a common mechanism in these cancers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology, pathogenesis, clinical presentation and management of TB in patients with HIV and diabetes.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Budget impact analysis of anakinra in the treatment of familial Mediterranean fever in Italy.

Journal of market access & health policy·2023
Same author

Assessment of trace element contamination and effects on Paracentrotus lividus using several approaches: Pollution indices, accumulation factors and biochemical tools.

The Science of the total environment·2023
Same author

Stress response to trace elements mixture of different embryo-larval stages of Paracentrotus lividus.

Marine pollution bulletin·2022
Same author

Effects of trace elements contaminations on the larval development of Paracentrotus lividus using an innovative experimental approach.

Aquatic toxicology (Amsterdam, Netherlands)·2022
Same author

Compositional and Morphological Characterization of 'Sorrento' and 'Chandler' Walnuts.

Foods (Basel, Switzerland)·2022

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The bax gene plays a critical role in initiating apoptosis, a process vital for preventing tumor development.
  • Alterations in the bax gene's promoter or coding sequences can impair its apoptosis-initiating function.
  • Understanding bax gene mutations is crucial for comprehending lymphomagenesis.

Purpose of the Study:

  • To investigate the frequency and nature of bax gene mutations in various types of B-cell lymphomas.
  • To determine if bax gene mutations contribute to the development of B-cell lymphomas by inhibiting apoptosis.

Main Methods:

  • Polymerase chain reaction-based single strand conformation polymorphism analysis (PCR-SSCP) and direct sequencing were employed.
  • The promoter region, all six exons, and flanking sequences of the bax gene were analyzed.

Related Experiment Videos

  • A cohort of 112 nodal and extranodal B-cell lymphomas was studied, including follicular lymphoma, mantle cell lymphoma, diffuse large B-cell lymphoma, splenic marginal zone B-cell lymphoma, and mucosa-associated lymphoid tissue (MALT) lymphomas.

    • Main Results:

    • Sequence alterations in the bax gene were detected in 11 out of 112 lymphoma cases.
    • Nine of these alterations were identified as polymorphisms, present in both tumor and non-tumor tissues.
    • Two cases exhibited tumor-specific point mutations in exons 3 and 6, altering the bax open reading frame.

    Conclusions:

    • Bax gene mutations that inhibit apoptosis are not a common mechanism in the pathogenesis of major types of nodal and extranodal B-cell lymphomas.
    • The findings suggest that other pathways are likely more significant in the development of these B-cell malignancies.