Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decorin is a Zn2+ metalloprotein.

V W Yang1, S R LaBrenz, L C Rosenberg

  • 1Graduate School of Biomedical Sciences, University of Texas, Houston Health Science Center, Houston, Texas 77030, USA.

The Journal of Biological Chemistry
|April 23, 1999
PubMed
Summary

Decorin, a proteoglycan found in mammalian extracellular matrix, binds tightly to zinc ions (Zn2+). This zinc binding influences decorin's structure and is mediated by its N-terminal domain.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Creative Health in practice: Hub-and-Spoke consortium working across West Yorkshire.

Perspectives in public health·2026
Same author

A randomized-controlled trial of parent-administered interventions to improve short-term motor outcomes in hospitalized very low birthweight infants.

Journal of neonatal-perinatal medicine·2024
Same author

Sexually transmitted infections: Prevalence and clinical outcomes among pregnant women in Western Sydney.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2024
Same author

Alkaline ceramidase 3 deficiency aggravates colitis and colitis-associated tumorigenesis in mice by hyperactivating the innate immune system.

Cell death & disease·2016
Same author

Protofilaments, filaments, ribbons, and fibrils from peptidomimetic self-assembly:  implications for amyloid fibril formation and materials science.

Journal of the American Chemical Society·2012
Same author

Multilayer vascular grafts based on collagen-mimetic proteins.

Acta biomaterialia·2011

Area of Science:

  • Biochemistry
  • Proteoglycan research
  • Extracellular matrix biology

Background:

  • Decorin is a ubiquitously distributed mammalian proteoglycan.
  • Its core protein features leucine-rich repeat motifs.

Purpose of the Study:

  • To investigate the potential for decorin to bind zinc ions (Zn2+).
  • To localize the Zn2+-binding sites within the decorin protein structure.

Main Methods:

  • Equilibrium dialysis was used to assess Zn2+ binding affinity.
  • Recombinant decorin N-terminal peptides were synthesized.
  • Circular dichroism spectroscopy analyzed structural changes upon Zn2+ binding.

Main Results:

  • Decorin exhibits high affinity for Zn2+.

Related Experiment Videos

  • Zn2+-binding sites are located in the N-terminal domain, containing cysteine residues.
  • A 41-amino acid peptide demonstrated full Zn2+ binding activity (KD of 3 x 10(-7) M).
  • Zn2+ binding induced secondary structure alterations in the peptide.

    • Conclusions:

    • Decorin's N-terminal domain possesses high-affinity Zn2+-binding capabilities.
    • Structurally related proteoglycans like biglycan may also bind Zn2+, unlike epiphycan and osteoglycin.