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Related Experiment Videos

Identification and study of tobacco mosaic virus movement function by complementation tests.

J G Atabekov1, S I Malyshenko, Morozov YuS

  • 1Department of Virology, Moscow State University, Russia. atabekov@virus.genebee.msu.su

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|April 23, 1999
PubMed
Summary

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Plant viruses can share movement proteins for cell-to-cell spread, a phenomenon called trans-complementation. This study explores how tobamoviruses complement other viruses with different movement protein gene structures.

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell-to-cell movement of plant viruses is crucial for infection spread.
  • Movement proteins (MPs) facilitate viral transport through plasmodesmata.
  • Tobamoviruses encode a single MP, while other viruses like PVX and BSMV utilize multiple proteins for movement.

Purpose of the Study:

  • To investigate the phenomenon of trans-complementation between plant positive-strand RNA viruses.
  • To analyze the complementation capabilities of tobamoviruses with viruses possessing different MP gene organizations.

Main Methods:

  • Double infections with movement-deficient and helper viruses.
  • Infections with recombinant viral genomes containing heterologous MPs.
  • Complementation assays in transgenic plants expressing specific MPs.

Related Experiment Videos

  • Co-bombardment of plant tissues with viral cDNAs and MP genes.

    • Main Results:

    • Demonstrated trans-complementation between tobamoviruses and viruses with triple block MP genes (PVX, BSMV).
    • Showcased the functional compatibility of different MP systems in facilitating viral movement.
    • Highlighted the versatility of tobamovirus MPs in complementing movement-deficient viruses.

    Conclusions:

    • Plant positive-strand RNA viruses exhibit functional complementation of cell-to-cell movement.
    • The MP of tobamoviruses can functionally substitute for the movement machinery of other virus groups.
    • This cross-complementation provides insights into the evolution and mechanisms of viral movement.