Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature modulated protein release from pH/temperature-sensitive hydrogels.

T G Park1

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, South Korea. tgpark@sorak.kaist.ac.kr

Biomaterials
|April 23, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activated Notch1 interacts with p53 to inhibit its phosphorylation and transactivation.

Cell death and differentiation·2006
Same author

Novel polymer-DNA hybrid polymeric micelles composed of hydrophobic poly(D,L-lactic-co-glycolic acid) and hydrophilic oligonucleotides.

Bioconjugate chemistry·2001
Same author

Microencapsulation of dissociable human growth hormone aggregates within poly(D,L-lactic-co-glycolic acid) microparticles for sustained release.

International journal of pharmaceutics·2001
Same author

The effect of charge increase on the specificity and activity of a short antimicrobial peptide.

Peptides·2001
Same author

Protein release microparticles based on the blend of poly(D,L-lactic-co-glycolic acid) and oligo-ethylene glycol grafted poly(L-lactide).

Journal of controlled release : official journal of the Controlled Release Society·2001
Same author

A new gene delivery formulation of polyethylenimine/DNA complexes coated with PEG conjugated fusogenic peptide.

Journal of controlled release : official journal of the Controlled Release Society·2001
Same journal

Oral colon-targeted micro-nano formulation engineered in microfluid for synergistic therapy of inflammatory bowel disease.

Biomaterials·2026
Same journal

Manganese@Gold cluster-coordinated covalent organic frameworks-based artificial metalloenzymes with cascade biocatalysis and amplified systemic stimulation to combat malignant tumor metastasis.

Biomaterials·2026
Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
See all related articles

This study developed a dual-responsive hydrogel for controlled insulin release. Temperature and thermal cycling significantly impacted release rates, while pH had minimal effect, offering insights into smart drug delivery systems.

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Controlled release of therapeutic proteins like insulin is crucial for managing diabetes.
  • Developing smart hydrogels that respond to physiological stimuli offers potential for improved drug delivery.
  • Existing hydrogels may lack responsiveness to multiple triggers, limiting precise control over release kinetics.

Purpose of the Study:

  • To synthesize and characterize a novel hydrogel matrix capable of dual-responsive (pH and temperature) controlled release of insulin.
  • To investigate the influence of environmental factors (pH, temperature, thermal cycling) on insulin release profiles from the synthesized hydrogel.
  • To evaluate the potential of this hydrogel as a platform for advanced insulin delivery systems.

Main Methods:

Related Experiment Videos

  • Synthesis of a hydrogel via copolymerization of N-isopropylacrylamide (temperature-sensitive) and N,N'-dimethylaminopropylmethacrylamide (pH-sensitive).
  • Entrapment of insulin crystals within the hydrogel matrix during the synthesis process.
  • Systematic investigation of insulin release kinetics under varying pH, temperature, and thermal cycling conditions.

Main Results:

  • The synthesized hydrogel demonstrated dual responsiveness, although pH sensitivity was less pronounced.
  • Insulin release rates were significantly influenced by temperature and repeated thermal cycling.
  • pH variations showed a negligible impact on the overall insulin release profiles from the hydrogel matrix.

Conclusions:

  • The developed N-isopropylacrylamide and N,N'-dimethylaminopropylmethacrylamide copolymer hydrogel shows promise for temperature-controlled insulin delivery.
  • Further optimization is needed to enhance pH responsiveness for a more finely tuned dual-stimuli system.
  • The findings highlight the critical role of thermal stimuli in modulating insulin release from this specific hydrogel formulation.