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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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Published on: January 16, 2012

Antibody purification methods

M A Vijayalakshmi1

  • 1Laboratoire d'Interactions Moléculaires et Technologie des Separations, CNRS-UPRES A 6022; Université de Technologie de Compiegne, France. viji@utc.fr

Applied Biochemistry and Biotechnology
|April 24, 1999
PubMed

Antibodies (Abs) from the sera of patients with autoimmune diseases are reported to have different catalytic functions. Their recovery by efficient purification methods is, therefore, a crucial step. This article reviews different available methods for their recovery and emphasizes a new approach, namely adsorbents with immobilized histidine, which allows a good purification both in yield and purity of Abs, with the additional advantage of using gentle elution conditions. This, in turn, will ensure the recovery of intact (nondenatured) catalytically functional Abs, directly from the sera.

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