Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oxidative DNA damage processing in nuclear and mitochondrial DNA.

V A Bohr1, G L Dianov

  • 1Laboratory of Molecular Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA.

Biochimie
|April 24, 1999
PubMed
Summary

Oxidative stress damages DNA, potentially causing cancer and aging. Contrary to popular belief, mitochondria possess efficient DNA repair mechanisms for this oxidative damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The cGAS-STING signaling pathway is modulated by urolithin A.

Mechanisms of ageing and development·2023
Same author

A POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrid accumulation.

Nature communications·2022
Same author

DNA Polymerase Beta Participates in Mitochondrial DNA Repair.

Molecular and cellular biology·2017
Same author

Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice.

Cell death & disease·2014
Same author

Associations of subjective vitality with DNA damage, cardiovascular risk factors and physical performance.

Acta physiologica (Oxford, England)·2014
Same author

Mitochondria, oxidative DNA damage, and aging.

Journal of the American Aging Association·2013

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Organisms face constant oxidative stress from internal and external sources.
  • Oxidative stress causes DNA modifications, leading to mutations, genomic instability, cancer, and aging.
  • Oxidative DNA lesions accumulate with age, primarily in mitochondrial DNA.

Purpose of the Study:

  • To investigate the mechanisms of DNA repair pathways.
  • To examine the repair of various oxidative DNA lesions.
  • To quantify steps within DNA repair processes in both nuclear and mitochondrial DNA.

Main Methods:

  • Utilized diverse experimental approaches to study DNA repair.
  • Focused on base excision repair (BER) and nucleotide excision repair (NER) pathways.
  • Differentiated DNA damage processing in nuclear versus mitochondrial DNA.

Main Results:

  • Identified complex DNA repair mechanisms for oxidative lesions.
  • Demonstrated efficient repair of oxidative DNA damage in mitochondria.
  • Quantified repair steps and lesion repair across different DNA types.

Conclusions:

  • Mitochondrial DNA repair is more robust than previously assumed.
  • Understanding DNA repair pathways is crucial for aging and cancer research.
  • Further clarification of BER and NER pathway interactions is warranted.

Related Experiment Videos