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Related Experiment Videos

2-oxo-3-hydroxy-LSD: an important LSD metabolite?

A G Verstraete1, E J Van de Velde

  • 1Laboratorium voor klinische biologie-Toxicologie, Universitair Ziekenhuis, Gent, Belgium.

Acta Clinica Belgica
|April 27, 1999
PubMed
Summary

2-oxo-3-hydroxy-LSD is found in urine at significantly higher concentrations than LSD, potentially improving drug detection times and screening confirmation. This metabolite is not conjugated and can be detected using GC-MS analysis.

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Area of Science:

  • Forensic Chemistry
  • Analytical Toxicology
  • Pharmacology

Background:

  • Previous research suggests higher urinary concentrations of 2-oxo-3-hydroxy-LSD compared to LSD.
  • Understanding LSD metabolite profiles is crucial for accurate drug testing.

Purpose of the Study:

  • To quantify and compare urinary concentrations of LSD and its metabolite, 2-oxo-3-hydroxy-LSD.
  • To evaluate the potential of 2-oxo-3-hydroxy-LSD as a biomarker for LSD use.

Main Methods:

  • Urine samples were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS).
  • Solid-phase extraction (SPEC.PLUS MP1 disks) and derivatization (BSTFA) were employed.
  • Quantification and confirmation ions (m/z 309 and 499) were used for 2-oxo-3-hydroxy-LSD-bis-TMS.

Main Results:

  • 2-oxo-3-hydroxy-LSD concentrations were 4 to 41 times higher than LSD concentrations in tested samples.
  • Measured 2-oxo-3-hydroxy-LSD levels ranged from 8021 to 28466 pg/mL.
  • The metabolite 2-oxo-3-hydroxy-LSD does not appear to be conjugated.

Conclusions:

  • 2-oxo-3-hydroxy-LSD is a major LSD metabolite in urine, found at significantly higher concentrations than LSD itself.
  • This metabolite could enhance the confirmation of positive drug screenings.
  • Elevated levels of 2-oxo-3-hydroxy-LSD may extend the detection window for LSD use.

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