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Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
Published on: June 25, 2013
The MobA-linked primase is the only replication protein of R1162 required for conjugal mobilization
1Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Journal of Bacteriology
|April 28, 1999
Abstract:
Cells newly transformed with plasmid R1162 DNA were used as donors in conjugal matings to determine if the plasmid replication genes are necessary for transfer. An intact system for vegetative replication is not required for transfer at normal frequency, but the plasmid primase, in the form linked to the nickase, must be present in donor cells.
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