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Related Experiment Videos

Changes in lectin binding patterns in rat esophageal epithelium during development.

W Fukawa1, H Kawakami, H Hirano

  • 1Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan.

Okajimas Folia Anatomica Japonica
|April 28, 1999
PubMed
Summary

Glycoprotein patterns in developing rat esophagus change significantly after 17 days post coitum. Lectin binding reveals distinct changes in esophageal epithelial cell differentiation.

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Area of Science:

  • Histology
  • Developmental Biology
  • Glycobiology

Background:

  • The esophageal epithelium undergoes significant differentiation during development.
  • Understanding molecular changes, particularly in glycoproteins, is crucial for characterizing this process.

Purpose of the Study:

  • To investigate changes in lectin binding patterns in rat esophageal epithelium during development.
  • To correlate these changes with the morphological differentiation of epithelial cell layers.

Main Methods:

  • Utilized light and electron microscopy to examine lectin binding.
  • Employed 15 types of biotinylated lectins for staining.
  • Observed changes in lectin reactivity from before 17 days post coitum (p.c.) to after 18 days p.c.

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Main Results:

  • Lectin staining was weak before 17 days p.c. and progressively positive thereafter.
  • Concanavalin A (ConA) and Wheat Germ Agglutinin (WGA) bound to all epithelial layers.
  • Dolphin Seaweed Agglutinin (DSA) bound to middle and surface layers, while Ulex Europaeus Agglutinin 1 (UEA-1) bound to the middle layer.
  • UEA-1 showed strong binding on cell membranes and Golgi membranes of middle-layer cells, and apical membranes of basal cells.
  • ConA and DSA exhibited positive reactions on cell, Golgi, and nuclear membranes.
  • Morphological differentiation of the three epithelial layers (basal, middle, surface) became apparent after 17 days p.c.

Conclusions:

  • Lectin binding patterns reflect dynamic changes in glycoprotein distribution during rat esophageal development.
  • These alterations in glycoproteins are closely associated with the differentiation of mucous epithelial cells.