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Related Experiment Videos

Decrease in mucosal alkaline phosphatase: a potential marker of intestinal reperfusion injury.

A C Sisley1, T R Desai, K L Hynes

  • 1Department of Surgery, University of Chicago, Illinois, USA.

The Journal of Laboratory and Clinical Medicine
|April 28, 1999
PubMed
Summary
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Alkaline phosphatase may serve as a specific marker for intestinal reperfusion injury. This enzyme

Area of Science:

  • Gastroenterology
  • Biochemistry
  • Surgical Research

Background:

  • Intestinal ischemia requires prompt blood flow restoration to prevent tissue damage.
  • Reperfusion following ischemia generates oxygen free radicals, causing further injury.
  • A specific marker to distinguish ischemia from reperfusion injury is currently lacking.

Purpose of the Study:

  • To evaluate alkaline phosphatase as a potential specific marker for intestinal reperfusion injury.
  • To assess enzyme activity changes in canine and human ex vivo ischemia/reperfusion models.

Main Methods:

  • Small bowels from canines and human organ donors were subjected to ischemia and reperfusion.
  • Brush border membrane enzymes (alkaline phosphatase, sucrase, maltase, gamma-glutamyl transpeptidase) were measured.

Related Experiment Videos

  • Enzyme activities in ischemic versus reperfused intestinal tissues were compared.

    • Main Results:

    • Warm ischemia alone did not alter enzyme activities in either model.
    • Alkaline phosphatase activity significantly decreased in both canine and human reperfusion models.
    • Activities of sucrase, maltase, and gamma-glutamyl transpeptidase remained unchanged post-reperfusion.

    Conclusions:

    • Alkaline phosphatase activity may serve as a specific indicator of intestinal reperfusion injury.
    • This enzyme's activity could enable quantitative assessment of therapeutic interventions for reperfusion injury.
    • Findings suggest potential for improved management of intestinal ischemia/reperfusion scenarios.