Microglial reaction in some CNS nuclei following nerves transection in BALB/c and interferon-gamma gene knockout mice

The present study was aimed to ascertain if the endogenous IFN-gamma is necessary for induction of microglial reaction associated with the CNS neurons after neurectomy of the vagus and hypoglossal nerves in IFN-gamma gene knockout mice and BALB/c mice serving as controls. Vigorous microglial reaction as detected by Mac-1 antibody was elicited in the brainstem nuclei in both strains of mice. Increased Mac-1 immunoreactivity in microglia was detected as early as 1 day post-operation in the ipsilateral dorsal nucleus of vagus, nucleus ambiguus and the hypoglossal nucleus; a similar feature was observed in the nucleus tractus solitarius bilaterally. Mac-1 immunoreactivity in reactive microglia appeared to increase with survival intervals and was comparable throughout in both strains of mice. It is therefore concluded that endogenous IFN-gamma is not responsible for upregulation of complement type 3 receptor immunomolecules in reactive microglia following nerves sectioning.