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The effect of mitomycin-C on the healing process in rabbit extraocular muscle surgery
1Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea.
Background And Objective:
One of the most serious complications following extraocular muscle surgery is formation of postoperative adhesions that may result in restrictive ocular motility dysfunction. The effect of mitomycin-C (MMC) on postoperative wound healing in extraocular muscle surgery was investigated.
Materials And Methods:
Sixteen albino rabbits (24 eyes) were used and anesthetized with ketamine and xylazine. These rabbits were divided into 3 groups; (1) control group (balanced salt solution (BSS) exposure for 5 minutes), (2) Group 1 (0.5 mg/ml MMC exposure for 1 minute) and (3) Group 2 (0.5 mg/ml MMC exposure for 5 minutes). After 3 mm recession of superior rectus muscle, MMC or BSS was applied using Weck-Cel sponge (Solan, Jacksonville, FL). The reduction of fibroblast and collagen fiber proliferation were evaluated histopathologically with light microscope at 1, 2, 4 and 8 weeks after operation. We also evaluated the amount of basic fibroblast growth factor (bFGF) by immunohistochemical study at the same time.
Results:
Histopathologically, there was a more significant reduction of fibroblast and collagen fiber proliferation in Group 1 and 2 than the control group. Immunohistochemically, Group 1 and 2 showed lesser expression of bFGF than the control group. But, there was no difference between Group 1 and 2.
Conclusions:
Topical intraoperative application of mitomycin C in extraocular muscle surgery may reduce postoperative adhesions.
Insights
Mitomycin-C (MMC) applied during extraocular muscle surgery significantly reduced fibroblast and collagen proliferation, potentially decreasing postoperative adhesions. This study investigated MMC
Area of Science:
- Ophthalmology
- Surgical Research
- Wound Healing
Background:
- Postoperative adhesions are a serious complication of extraocular muscle surgery.
- These adhesions can lead to restrictive ocular motility dysfunction.
Purpose of the Study:
- To investigate the effect of mitomycin-C (MMC) on wound healing after extraocular muscle surgery.
- To evaluate MMC's potential in reducing postoperative adhesions.
Main Methods:
- Albino rabbits underwent superior rectus muscle recession.
- Eyes were treated with either balanced salt solution (control) or mitomycin-C (MMC) at varying concentrations and durations.
- Fibroblast and collagen proliferation, and basic fibroblast growth factor (bFGF) expression were assessed histopathologically and immunohistochemically.
Main Results:
- Both MMC treatment groups showed a significant reduction in fibroblast and collagen fiber proliferation compared to the control group.
- Lesser expression of bFGF was observed in the MMC-treated groups.
- No significant difference in outcomes was found between the different MMC exposure durations.
Conclusions:
- Topical intraoperative application of mitomycin-C may effectively reduce postoperative adhesions in extraocular muscle surgery.
- MMC demonstrates potential as an adjunct therapy to improve surgical outcomes.