Related Experiment Video
Updated: Jul 17, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Characterization of peroxidase:anti-peroxidase immune complexes by capillary zone electrophoresis and
1Department of Immunology, Sidney Kimmel Cancer Center, San Diego, CA 92121, USA.
Abstract:
Determination of the molecular constituents of commercial peroxidase:anti-peroxidase (PAP) preparations is necessary for the proper interpretation of PAP applications based on competitive binding assay. Capillary zone electrophoresis with field 300 V/cm, 40 cm capillary length (20 cm effective length), and high-performance size exclusion chromatography equipped with Superose 12 HR10/30 column revealed that a PAP preparation used for Fc gamma receptor studies contained multiple sizes of immune complexes, an excess amount of free peroxidase, and little or no free anti-peroxidase antibody. The antibody:antigen ratios of the three major immune complex components were 2:2, 1:2, and 1:1. These techniques provide useful methods of qualitative, as well as quantitative analysis of PAP preparations.
Related Concept Videos
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Affinity Chromatography
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

