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Related Experiment Videos

Fully automated analytical method for codeine quantification in human plasma using on-line solid-phase extraction and

J A Pascual1, J Sanagustín

  • 1Pharmacology Research Unit, Municipal Institute of Medical Research, IMIM, Barcelona, Spain.

Journal of Chromatography. B, Biomedical Sciences and Applications
|April 29, 1999
PubMed
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A new, automated method accurately analyzes codeine in human plasma using solid-phase extraction and UV detection. This sensitive technique offers precise quantification for clinical and forensic applications.

Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Clinical Toxicology

Background:

  • Accurate quantification of codeine in biological matrices is crucial for therapeutic drug monitoring and forensic analysis.
  • Existing methods for codeine analysis may lack automation, sensitivity, or efficiency.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and fully automated analytical method for codeine determination in human plasma.

Main Methods:

  • Online solid-phase extraction (SPE) with a C2 cartridge for sample clean-up.
  • Isocratic elution with mobile phase recirculation for improved reproducibility.
  • High-performance liquid chromatography (HPLC) with UV detection at 212 nm.
  • Oxycodone as the internal standard (I.S.).

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Main Results:

  • The method achieved a low detection limit of 0.5 µg/L for codeine.
  • High inter-assay precision (5.03% RSD) and accuracy (1.82% RE) at 2 µg/L.
  • A wide calibration range of 2-140 µg/L was established.
  • Separation of codeine, its metabolite norcodeine, and internal standard oxycodone was achieved within 10 minutes.

Conclusions:

  • The developed automated method is suitable for the routine analysis of codeine in human plasma.
  • The method's simplicity, sensitivity, and automation make it valuable for clinical and forensic laboratories.