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Related Experiment Videos

cDNA sequence coding for the alpha'-chain of the third complement component in the African lungfish.

A Sato1, H Sültmann, W E Mayer

  • 1Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.

Scandinavian Journal of Immunology
|April 29, 1999
PubMed
Summary

Sequencing the African lungfish C3 alpha-chain reveals its evolutionary position among vertebrates. This finding aids in understanding the origin of tetrapods and the complement system

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Area of Science:

  • * Molecular biology
  • * Evolutionary biology
  • * Immunology

Background:

  • * The complement system is a crucial part of innate immunity in vertebrates.
  • * Complement component 3 (C3) plays a central role in complement activation.
  • * Understanding C3 evolution provides insights into vertebrate evolution.

Purpose of the Study:

  • * To sequence and characterize the C3 alpha-chain from the African lungfish (Protopterus aethiopicus).
  • * To analyze the evolutionary relationships of lungfish C3 within jawed vertebrates.
  • * To investigate the implications of C3 structure for tetrapod origins.

Main Methods:

  • * Sequencing of cDNA clones encoding the lungfish C3 alpha-chain.
  • * Bioinformatic analysis of the deduced amino acid sequence.

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  • * Comparative sequence analysis with C3 from other vertebrates.

    • Main Results:

    • * The lungfish C3 alpha-chain sequence was determined, suggesting an alpha:beta dimer structure.
    • * Conserved functional sites for activation, inactivation, and properdin binding were identified.
    • * Sites for complement receptor 2 and factor H interaction showed poor conservation.

    Conclusions:

    • * Lungfish C3 shares structural similarities with other jawed vertebrates, reflecting conserved functions.
    • * Divergent binding sites for complement receptor 2 and factor H suggest potential differences in complement regulation.
    • * The C3 alpha-chain sequence provides valuable data for resolving the evolutionary origin of tetrapods.