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Related Experiment Videos

Arsenic binding proteins from human lymphoblastoid cells.

D B Menzel1, H K Hamadeh, E Lee

  • 1Department of Community and Environmental Medicine, University of California, Irvine 92697-1825, USA. menzel@uci.edu

Toxicology Letters
|April 30, 1999
PubMed
Summary

Researchers identified proteins that bind to toxic inorganic arsenic (AsIII) in human cells. These arsenic-binding proteins may play a role in arsenic toxicity and its biological actions.

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Area of Science:

  • Toxicology
  • Molecular Biology
  • Biochemistry

Background:

  • Arsenic is a widespread contaminant in drinking water and food.
  • The precise mechanisms underlying inorganic arsenic toxicity remain largely unknown.
  • Understanding arsenic's molecular interactions is crucial for assessing health risks.

Purpose of the Study:

  • To isolate and identify proteins that bind to arsenite (AsIII), the toxic form of arsenic.
  • To investigate the induction of arsenic-binding proteins in human cells exposed to arsenite.
  • To explore the potential role of these proteins in arsenic's biological effects.

Main Methods:

  • Human lymphoblastoid cells were treated with arsenite (AsIII).
  • Proteins with high affinity for AsIII were isolated using a p-aminophenylarsine oxide affinity column.

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  • Proteins were eluted using varying concentrations of 2-mercaptoethanol and identified by molecular weight and preliminary analysis.

    • Main Results:

    • At least four arsenic-binding proteins (50, 42, 38.5, and 19.5 kDa) were isolated.
    • Tubulin and actin were tentatively identified among the arsenic-binding proteins.
    • Heme oxygenase 1 was induced by AsIII but did not bind to the affinity column, indicating variable arsenic interactions.

    Conclusions:

    • Arsenite (AsIII) induces multiple proteins with varying affinities for arsenic in the +3 oxidation state.
    • These arsenic-binding proteins may act as high-affinity sites for AsIII.
    • The identified proteins could be involved in the toxic mechanisms of inorganic arsenic.