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Determination of cyclosporin A in 20% ethanol by a magnetic beads-based immunofluorescence assay
M V Kiselev1, A K Gladilin, N S Melik-Nubarov
1Chemistry Department, Moscow State University, Moscow, Russia.
Analytical Biochemistry
|May 1, 1999
Abstract:
A rapid magnetic beads-based immunoassay for the immunodepressant drug cyclosporin A (CsA) has been developed. The method allows CsA determination in medium with a higher content of ethanol compared to conventional immunochemical techniques due to increased antibody stability. Monitoring of the drug in ethanol extracts from patient's whole blood without many-fold dilution with aqueous buffer is possible. The assay has adequate specificity and sensitivity for CsA to be suitable for the routine monitoring of therapy.