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Related Experiment Videos

Rhinoviruses replicate effectively at lower airway temperatures.

N G Papadopoulos1, G Sanderson, J Hunter

  • 1University Department of Medicine, University of Southampton, United Kingdom.

Journal of Medical Virology
|May 1, 1999
PubMed
Summary

Rhinoviruses, linked to asthma attacks, can replicate in the lower airways. Different rhinovirus serotypes show varying temperature preferences, with some replicating well at 37°C, challenging the notion that lower airway infection is impossible.

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Area of Science:

  • Virology
  • Respiratory Medicine
  • Immunology

Background:

  • Rhinoviruses are a major cause of asthma exacerbations.
  • The ability of rhinoviruses to infect the lower airways is debated due to their optimal replication temperature of 33°C (nasal passages).
  • Previous studies used only one rhinovirus serotype, limiting generalizability.

Purpose of the Study:

  • To investigate temperature preferences for rhinovirus replication across multiple serotypes.
  • To determine if rhinoviruses can replicate effectively at lower airway temperatures (37°C).
  • To assess the potential for rhinovirus infection in the lower airways.

Main Methods:

  • Titration of eight rhinovirus serotypes (1b, 2, 7, 9, 14, 16, 41, 70) in Ohio-HeLa cells at 33°C and 37°C.

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  • Continuous culture of viruses at both temperatures for 2-4 passages, followed by titration.
  • Culture and RT-PCR analysis of wild-type rhinoviral samples from nasal aspirates at 33°C and 37°C.

    • Main Results:

    • Most rhinovirus serotypes and wild-type viruses showed slightly better replication at 33°C than at 37°C.
    • However, significant viral titers were achieved at 37°C, sufficient to initiate infection.
    • Some serotypes demonstrated equal or even enhanced replication at 37°C compared to 33°C.

    Conclusions:

    • Rhinovirus temperature preferences vary among serotypes.
    • Replication at 37°C is sufficient to support lower airway infection.
    • Temperature is unlikely to be a prohibitive factor for rhinovirus infection in the lower airways during asthma exacerbations.