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Genetic determinants of 5-lipoxygenase transcription
1Division of Pulmonary and Critical Care Medicine, Departments of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, MA, USA. jdrazen@rics.bwh.harvard.edu
Background:
5 Lipoxygenase (5-LO) is a critical enzyme in the production of the leukotrienes. We have identified a series of mutations in the 5-LO gene that modify gene transcription. These mutations consist of addition of an Sp-1 binding motif (-GGGCGG-) or deletion of one or two Sp-1 binding motifs in the 5-LO core promoter.
Methods:
Mutant forms of the 5-LO core promoter were placed in a chloramphenicol acetyl transferase (CAT) reporter construct using either HeLa or SL-2 cells.
Results:
In HeLa cells all of the mutant forms are less effective in driving CAT reporter activity than the wild-type promoter. In SL-2 cells the construct containing the addition mutation was more effective in driving CAT reporter activity, while the constructs containing the deletion mutations were less effective.
Conclusions:
These data indicate that naturally occurring mutations in the 5-LO core promoter modify gene transcription in vitro.