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Related Experiment Videos

Strand-specific compositional asymmetries in double-stranded DNA viruses.

A Grigoriev1

  • 1Max-Planck-Institute for Molecular Genetics, Berlin, Germany. andrei.grigoriev@gpc-ag.com

Virus Research
|May 4, 1999
PubMed
Summary

DNA virus genomes exhibit significant strand compositional asymmetries, with GC skew differing between leading and lagging strands, often switching near replication origins. These findings offer insights into viral genome replication and evolution.

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Area of Science:

  • Virology
  • Genomics
  • Molecular Biology

Background:

  • Viral genomes display compositional biases.
  • Understanding these biases is crucial for deciphering replication strategies.

Purpose of the Study:

  • To analyze compositional asymmetries in double-stranded DNA virus genomes.
  • To investigate the relationship between GC skew and replication/transcription.

Main Methods:

  • Analysis of 22 complete double-stranded DNA virus genome sequences.
  • Examination of GC skew on leading/lagging and transcribed/non-transcribed strands.
  • Use of cumulative skew plots for genome comparison.

Main Results:

  • Striking compositional asymmetries observed between leading/lagging and transcribed/non-transcribed strands.

Related Experiment Videos

  • GC skew polarity typically switches near replication origins in bi-directionally replicated genomes.
  • Linear GC skew change in adenovirus; distinct GC skew patterns in papillomavirus related to replication/transcription direction.

    • Conclusions:

    • Replication and transcription processes, along with repair mechanisms, likely contribute to observed strand asymmetries.
    • GC skew analysis provides a valuable tool for comparing viral genomes and understanding their replication dynamics.