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Binding of Haemophilus ducreyi to extracellular matrix proteins
1Departments of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. mebauer@iupui.edu
Infection and Immunity
|May 4, 1999
Abstract:
We developed an enzyme-linked immunosorbent assay-based assay to assess Haemophilus ducreyi binding to extracellular matrix (ECM) proteins. H. ducreyi 35000HP bound to fibronectin, laminin, and type I and III collagen but not to type IV, V, or VI collagen or elastin. Isogenic strains with mutations in ftpA or losB bound as well as the parent, suggesting that neither pili nor full-length lipooligosaccharide is required for H. ducreyi to bind to ECM proteins.